Fluidigm Juno - Loading

Juno System: User Guide
Appendix A: Troubleshooting
Loading
48
Loading
Observation Possible Cause Recommended Action
• No or low vacuum.
Message:
“Insufficient vacuum.
Clean the thermal
chuck or load an IFC,
and try again.”
• Message: “Load
error”
Dirty IFC or chuck
surface
• Clean the chuck surface and the back of the IFC with
70% ethanol or 70% isopropyl alcohol and a lint-free
cloth. (See Clean the Thermal Cycler Chuck on
page 50.) Reload the IFC.
• Insert the IFC when loading for a run.
• Carrier orientation
error. Message: “The
IFC is in the wrong
orientation.”
• Message: “Load
error”
IFC placed incorrectly
on the tray
Place the ejected IFC correctly on the tray. The notched
corner of the IFC should align with the white notch on the
tray.
Barcode read error.
Message: “Enter loading
fixture barcode.”
Optical character
recognition failed to
read barcode on IFC or
the Interface Plate
Loading Fixture
• Ensure that the IFC barcode faces you.
• Enter barcode manually in the Enter IFC barcode
screen.
Load error. Message:
“The carrier is not
detected.”
• Interface plate is not
correct for the IFC
• Interface plate not
supported on system
• Interface plate
missing
• Switch or install the interface plate with an interface
plate supported on the Juno system and that supports
the IFC. (See Components of the Juno System on
page 8, Install an Interface Plate on page 29, and
Switch an Interface Plate on page 26.) If necessary, tap
OPEN to remove the incorrect IFC, then re-load the
correct IFC by tapping LOAD.
• Position the ejected IFC and interface plate correctly.
Ensure that the notched corner of the IFC aligns with
the white notch on the tray. Insert the interface plate in
the direction of the arrows with THIS SIDE UP facing up.
Confirm that the loading map sticker has been removed
from the bottom of the IFC.
Message:
“Unrecoverable error.”
Various • Restart the system.
• If the problem persists, contact Fluidigm technical
support.