Juno System: User Guide
7
Chapter 1: Introducing the Juno System
The Juno System
The Juno™ system is used for genotyping low-concentration DNA with the Juno 96.96
Genotyping IFC (integrated fluidic circuit) and for preparing libraries for targeted sequencing
with the LP 192.24 and LP 48.48 IFCs. This is possible through advanced microfluidics
technology.
In a single workflow, the Juno 96.96 Genotyping IFC integrates preamplification and
genotyping reactions of up to 96 samples and 96 genotyping assays to produce 9,216
genotypes in less than three hours. Samples are loaded into individual inlets of the Juno
96.96 Genotyping IFC, then distributed across multiple reaction chambers in nanoliter-
volume aliquots. With high-quality samples, detecting the specific targets requires a sufficient
number of copies in the final preamplification mix of 4 μL. After genotyping is performed on
the Juno system, the IFC is scanned on the EP1™ system or Biomark™ HD system to collect
genotyping data for later analysis.
The LP 192.24 IFC facilitates parallel amplification of up to 192 samples and up to 2,400
assays for targeted sequencing. The LP 48.48 IFC facilitates parallel amplification of up to 48
samples and up to 4,800 assays for targeted sequencing. Juno performs sample and assay
loading, mixing, thermal cycling, and harvesting automatically for further processing and
targeted sequencing.
NOTE For Research Use Only. Not for use in diagnostic procedures.
Consumables Ordering
To reorder IFCs and reagents, contact your regional Fluidigm sales representative or
distributor. Go to fluidigm.com/contact.html.
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