Reagent Name. Contains the name of the reagent.
Chip Serial Number.* Contains RFID chip serial number.
Chip Type. Click the Chip Type list and select the desired chip type (Low Volume, High
Volume, Low Volume PFE, High Volume PFE, or HV Continuous Flow) that you want to
use. The actual chip should match the chip type selected.
Input Source Type. Click the Input Source Type list and select the desired input source
type (Tube or Pipette Tip) for your experiment. The recommended length for tubing is 450
mm. The HV and LV chips use a slightly different tubing. The HV tubing is green while the
LV tubing is white. Please make sure you have matched the tube and chip accordingly.
Prime Volume. Type the desired volume to fill your tubing and/or chip channels in the
Prime Volume box. The MANTIS typically primes the chip only once at the beginning of
the dispense to make sure all of the liquid channels are full. You will know the channels
are full once reagent starts dispensing from the chip nozzle.
Pre-Dispense Volume. Type the desired reagent volume that you would like to dispense
to waste before dispensing into your plate in the Pre-Dispense Volume box.
Recovery Volume. Type the desired reagent volume that you would like to return to your
source container (pipette tip or tube) in the Recovery Volume box.
Wash Step(s). Select the wash steps you want to execute.
● 1 (Station 1) — This option enables MANTIS to wash the chip using only the wash
liquid available in Wash Station 1.
● 2 (Station 1-2) — This option enables MANTIS to wash the chip using liquid in Wash
Station 1 and rinse the chip using wash liquid in Wash Station 2.
● 3 (Station 1-2-1) — This option enables MANTIS to do the 3-step wash. You must fill
the Wash Station 1 with water and Wash Station 2 with wash solution. MANTIS will
wash the chip using water in the Wash Station 1, then wash solution in Wash Station 2
and rinse the chip using water in Wash Station 1. For more information, see Sterilizing
Mantis Chips on page 108.
Wash Volume 1 (Station 1). Type the desired wash volume to use during the chip cleaning
process.
Wash Volume 2 (Station 2). Type the desired wash volume to use during the chip cleaning
process.
Wash Volume 3 (Station 1). Type the desired wash volume to use during the chip cleaning
process.
Recovery Duration.** Type the recovery duration in milliseconds in the Recovery Duration
box.
Double Recovery.** Set this option to True to enable two reagent recovery cycles after
dispensing with the Continuous Flow chip.
Delay Between Recovery.** Type the delay time between two reagent recoveries in
milliseconds in the Delay Between Recovery box.
Pause for Reagent Refill.*** Enable this option to make the MANTIS pause during the
dispense to refill the pipette tip.
MANTIS User's Guide | Chapter 7: Software Overview
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