Once you have set up the hardware, you will use the MANTIS control software to either open
a dispense list or design a dispense.
Prerequisites
● You have at least one plate type already set up in the software.
To design a dispense:
1. Choose from the following options:
● Create a new dispense list: Click the New Dispense List button . Select a
plate type and click OK.
OR
● Open an existing dispense list: Click the Open Dispense List button . Find
your dispense list and click Open, then proceed to step 5.
2. Add a reagent to the dispense list. Define the Reagent Name and Dispense
Location and click OK. If you want to add additional reagents, click Add More.
3. Begin designing your plate dispense with the plate designer:
● Click and drag your mouse over a group of wells to select them. or select a
pattern from the Selection group under the plate designer.
● For a constant volume, type in a volume and press Enter on your keyboard.
● To use a gradient design, click the small down arrow next to the grid
button . You may choose a horizontal gradient, a vertical gradient, a
forward diagonal gradient, a backward diagonal gradient, checkerboard 1, or
checkerboard 2.
● To use a checkerboard dispense pattern, click the small down arrow next to the
grid button . You may choose checkerboard 1 or checkerboard 2.
● To add a backfill volume, click the small down arrow next to the grid button,
and then click the backfill volume button.
4. Click the Single Update Input button next to each reagent input. Select
the Chip Type (Low Volume, High Volume, High Volume PFE, Low Volume PFE, or
High Volume Continuous Flow chip), and type the Prime Volume (optional), Primary
Wash Volume, Secondary Wash Volume (optional), Recovery Volume, and Pre-
Dispense Volume. See Default Volume and Tubing Length Recommendation on
page 28.
Designing a Multi-Plate Dispense
Note: If the dispense list has more reagents than the amount of the chip stations,
the Assign To Input button will not work. The amount of reagents should be less
or same as the number of chip stations.
Note: You can skip these steps if you have loaded experiment designs
from FORMULATOR.
MANTIS User's Guide | Chapter 9: Dispensing With the MANTIS
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