Eliminate switch time
If the simultaneous opening of two valves as described above needs to be avoided, valve
switching may be performed sequentially by stopping the pump operation, closing the
first valve and opening the second valve.
Follow the instruction below to eliminate the switch time.
ActionStep
Stop the system pump.1
Close the valve to be closed.2
Open the valve to be opened.3
Start the system pump.4
Check column
Check that the correct column is used and that the column tubing is fitted correctly.
Check that the column has been equilibrated (if this is not included in the method).
Check flow kit
Check that the flow kit is of the same type as that entered in the System setup dialog
box in UNICORN Manager in UNICORN 5 or in the Edit System dialog box in UNICORN
7, see Before starting, on page 82.
Calibrate flow meter
It is recommended that the flow meter is always calibrated when the flow kit has been
changed.
The flow meter must be calibrated when changing to a different size flow kit.
Note:
To calibrate the flow meter, fill the flow kit with water. Perform the calibration as outlined
in Calibrating flow meter, on page 187.
ÄKTA ready Operating Instructions 28960345 AD 173
11 Run a method
11.1 Final preparations before a run
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