Multitron - Operating Manual
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20 August 2020 Page 39 of 158
4.2 Direct Steam Humidification
Part of the medium can evaporate, in particular in case of small vol-
umes of work (e.g. when using microtitre and deep well plates) and
long cultivation processes. To reduce evaporation, the device can be
fitted with a direct steam humidification. This regulates the humidity
in the incubation chamber to a defined setpoint and thus ensures a
consistent culture volume.
4.2.1 Setup and Function
The direct steam humidification is mounted on the rear of the casing.
A
controlled pump feeds water dropwise into an evaporator chamber
via a feed hose. The water evaporates and is then fed into the incu-
bation chamber.
There are three possibilities to supply the water:
Unpressurised from a water tank
From a pressurised pipe with inlet pressure of max. 0.3 bar
From a pressurised pipe with inlet pressure of max. 6 bar in
combination with the pressure elimination unit (see chapter
4.3 "Pressure Elimination Unit for Direct Steam Humidifica-
tion", page 42)
To prevent condensation inside the device, the device features several
heating elements (door heating
, door seal heating and floor heating).
These are activated automatically, as soon as the direct steam humid-
ification is switched on.
For a device without cooling on top, the device is additionally
equipped with a cover (H = 40 mm) to reduce condensation on the
ceiling.
The humidity in the incubation chamber is measured and regulated
using a humidity sensor. This is located in the rear wall of the incuba-
tion chamber.
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