3030226 Rev. D 5-27 Routine Maintenance
5.5.2 Endotoxin Testing Procedure
Due to the nature of endotoxin testing, it is recommended to consult with a laboratory
or test kit instructions for appropriate procedures.
Materials Required:
• Bucket or similar container
• Sterile syringe (30 mL minimum – 2 required)
• Sterile specimen, your lab may require.
1. Turn on the RO. Allow the RO to run for 10-15 minutes.
2. Using alcohol, wipe the sample port and allow to air dry. Using aseptic
technique, insert the male luer end of the first sterile/pyrogen free syringe into
the port, withdraw the syringe plunger to the maximum sample volume.
Remove and discard the syringe.
3. Using the second sterile/pyrogen free syringe draw a sample by inserting the
male luer end into the port and withdraw the appropriate sample as required
for your laboratory. Aseptically transfer the sample to the lab supplied
container, cap the specimen container immediately.
4. Label the specimen appropriately with:
• Test to be performed - "endotoxin"
• Sample source - product water/RO, machine serial number and room
location
• Time and date sample obtained
• Person who obtained specimen
• Any other pertinent information or procedures your facility or lab
requires
5. The samples should be assayed within 4 hours of collection or refrigerated
immediately and assayed within a 24-hour period.
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