6.4-6
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
Upon completion of the analysis, follow the shutdown procedure described in Section 4. Store the column in
RG019.
Excessive flushing will require an equally excessive re-equilibration when you start up again.
Note: The automatic valves prevent reagents from back-flowing onto the column. The inert gas should be left on
to preserve the OPA reagent.
Precautions to be Aware of in Glyphosate Analysis
Always wear gloves during the preparation of the reagents. The OPA and Thiofluor™ can cause skin irritation.
The OPA reagent is sensitive to air oxidation, degrades over time, and should be prepared fresh for optimum
sensitivity. OPA reagent is stable for at least one week when pressurized with inert gas.
Thiofluor
™
is extremely hygroscopic. Always keep in a tightly closed container.
The preparation of the OPA Diluent by the user is not recommended because sodium borate (any grades)
contains excessive amounts of heavy metal contaminants and insoluble matter. These impurities will eventually
precipitate in the reactor and flowcell. The one year warranty does not cover damage caused by these
contaminants.
Use Restore
™
if Iron contamination of the column is suspected.
Figure 6.4-E
5
10 15
min
Glyphosate
AMPA
Broccoli sample spiked with Glyphosate and AMPA, 50 ppb, 100 μl injection
To Next Page
Loading...