Pickering Laboratories Pinnacle PCX - Check Valve; Column Connections and Column Oven

2.3
Section 2
Overview
Pinnacle Operators Manual
Pickering Laboratories Inc.
Check Valve
The column check valve is located just outside of the
column oven (figure 2-3). The check valve is designed
to protect the column against backflow of the reagents in
case of unexpected HPLC shutdown.
Column Connections and Column Oven
The column oven contains the analytical column and
guard column (figure 2-3). The oven is a convection air
oven. The column heater utilizes re-circulating air flow
technology to provide quick, uniform column heating.
Fast column cooling is assisted by the introduction of
fresh air flow into the chamber by means of two fans
that are controlled by the Pinnacle PCX control software.
The temperature range holds within ± 1˚C resolution
from 5˚C above ambient to 75˚C. A temperature gradient
can be performed on the column to effect an increase
in the speed of the separation. The temperatures can
be programmed for a gradient with as many steps as
required for fine-tuning an analysis.
The heater block is designed to receive the analytical
column (5, 10, 15, and 25 cm long) and the guard
column when they are connected via a column easy-
connect fitting (included with the instrument, and sold
separately by Pickering, Cat. #3102-3064).
The outlet end of the column hangs in the slot at the top
of the column oven. The inlet to the guard column is at
the bottom of the column oven door.
The column oven door contains a pocket to fit around
the analytical column and guard. There is a groove in
the door that is lined with Santoprene foam rubber cord
that seals the door to the chassis and provides a stable
insulated heating environment.
The last part of the lead-in capillary is located inside the column oven to preheat the eluant for a more uniform
temperature within the column.
Figure 2-3
Column outlet tubing
Column oven door
Rubber foam seal
Analytical column
Inlet end
from HPLC
Check Valve
(3106-1007)
PEEK Nut
(1452-0226)
PEEK Nut
(3101-0011)
Blue Peek Tubing
to Guard
Column
Coupler/column connector
Guard column
Check valve