3.15
Section 3
InstallatIon
Pinnacle Operators Manual
Pickering Laboratories Inc.
Do four runs of the Amino Acid Standard that was provided with the chemical kit. Inject 10μl.
Discard the first injection.
Compare the chromatogram with that of the QC test of the system and column.
Verify that the system is functioning by using the IQ/OQ documents as reference (See Operation Manual,
Appendix)
For Carbamate and Glyphosate Analyses:
Set the detector excitation wavelength to 330 nm and the emission wavelength to 465 nm
Do at least four runs of the appropriate test mixture. Inject 10ul.
Discard the results of the first injection.
Compare the chromatogram with that of the QC test of the system and column.
Verify that the system is functioning by using the IQ/OQ documents as reference (See Operation Manual,
Appendix)
Shutdown
Upon completion of the analyses, use one of the following two procedures to shut down the Pinnacle PCX
system properly. These procedures can prevent potential column damage, reaction coil blockage, high
background fluorescence, reagent precipitation, or other problems.
The Pinnacle PCX must be flushed out at the end of a series of injections, and the reactors must be cooled
down. If the instrument is simply stopped, with reagent inside the reactor in a hot state, with no movement, then
the heated reactor will become blocked. It is very important for a long useful life that the reactors be flushed
out until the temperature is cool.
MANUAL SHUTDOWN
You may shutdown the Pinnacle PCX manually by pressing/selecting the Disable function in the Control menu
of the PCX control software.
If you choose this function, allow the HPLC to pump for at least 30 minutes to allow for the reactor to cool and
to flush reagent from the reactor.
OR
AUTOMATIC SHUTDOWN
Create a Shutdown Method for Pinnacle PCX according to section 4 of this manual. Set it up as the last
method in the Pinnacle PCX sequence. Make sure pump flow rate is set to 0 mL/min and reactor temperature
is set close to room temperature. You can leave the column at the operating temperature or set it at room
temperature.
Create corresponding slowdown Method for HPLC. Set it up as the last method in the HPLC sequence. Make
sure HPLC flushes column and reactor with column storage eluant for at least 30 min.
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