3.14
Section 3
InstallatIon
Pinnacle Operators Manual
Pickering Laboratories Inc.
Running a Chromatogram
Look in the column box for the gradient program and conditions.
Set the HPLC pump to run the starting eluant conditions, and while the system is equilibrating, set up the
gradient method in the HPLC software. We recommend that equilibration is set up at the end of each run (as a
post-run, if possible). Equilibration time should be at least 5 min long to give Pinnacle PCX enough time to refill
between the runs. If using relays to synchronize with Pinnacle PCX set up relay signal at time 0.0 in the HPLC
method. Contact your HPLC support representative if you have questions about setting up relay signal.
Create a sequence for the HPLC system.
Set up method and sequence for Pinnacle PCX (refer to section 4 OPERATION of this manual). Load the
sequence. Make sure correct sequence name and method name are displayed in the status bar at the bottom of
the Pinnacle software (If sequence and method fail to load: close Pinnacle PCX software, reopen Pinnacle PCX
software, reload sequence and method).
In the Pinnacle PCX software, select Enable in the Control menu. Column and reactor heater will be turned
ON. Wait for temperatures to reach the set point.
Select Refill pump(s) in the Control menu. Select Both pumps for 2-pump system. The valve(s) will move
to reagent (3-4) position and the pump(s) will refill enough reagent for one run.
NOTE: Pinnacle software will calculate needed reagent volume based on your run time and reagent flow rate.
Some extra volume will be added to that to ensure pumps will not run out of reagent during operation.
Select Pump(s) ON in the Control menu. Select Both pump(s) for 2-pump system. The valve(s) will move
to reactor (1-2) position and pump(s) will start dispensing reagent at set flow rate. Wait until the pressures are
stable.
Select Sequence – Start Sequence. Run time (0.0) will be displayed in the Status Bar at the bottom of the
Pinnacle PCX software. The reagent pump(s) will start dispensing reagent. Pinnacle now waits for injection
signal from HPLC in order to start running the method.
For TRIONE
®
Amino Acid Analysis:
Set the detector wavelength(s) to 570 and 440nm (if applicable).
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