3.13
Section 3
InstallatIon
Pinnacle Operators Manual
Pickering Laboratories Inc.
Reservoirs should be labeled with an appropriate label using the GLP of the laboratory.
Fill the Flush reservoir with water or 80-20 water/alcohol (IPA or Methanol)
Fill the Wash reservoir with 90-10 water/alcohol (IPA or Methanol).
Change Wash and Flush solutions at least once a week to prevent contamination.
Rinse the Reagent reservoir(s) with a small amount of reagent or diluent, and then if necessary prepare the
post-column reagents as described in the appropriate Application section of this manual.
FLUSH RESERVOIR CONNECTIONS
Take the 1/8" FEP tubes leading from the Flush (No. 5) position on the valves and slide the tubing through the
1/8" openings on the proper cap. This will be a snug fit to prevent slipping.
(PISTON) WASH RESERVOIR CONNECTIONS
Take the 1/4" C-flex tubing leading from the piston wash of the reagent pump(s) and slide it through the 1/4"
opening in the proper cap. This will be a snug fit to prevent slipping.
Reagent Pump Preparation
All of the reservoirs should now be filled with the appropriate solutions.
Ensure that the Flush and Piston Wash lines are immersed in liquid.
The Pinnacle PCX has a maximum of two waste lines that must be fed into a waste container. They can either be
fed into a separate container, or into the waste stream of the HPLC. These are the 1/8" OD clear FEP tubes that
are connected to the Waste port (No. 6) on the valves.
Dual pump systems will have two lines, and single pump systems will have one.
In the Pinnacle PCX software, select Empty pump(s) under the Control menu. Select Both pumps for
2-pump system. The valve(s) will move to waste (1-6) position and pump(s) will dispense all the liquid.
In the Pinnacle PCX software, select Flush pump(s) under the Control menu. Select Both pumps for
2-pump system. The system will flush pumps using Flush solution. The pump(s) will be empty at the end of
Flush cycle.
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