1.4
Section 1
IntroductIon
Pinnacle Operators Manual
Pickering Laboratories Inc.
An analogy will help us understand the concept. Let us use a river as an example. If it rains; the river swells.
If it stops raining; the level goes down. As the level fluctuates, it is equivalent to a periodic noise. To obtain a
constant flow, we need to add a reservoir (pulse dampener) and a dam (column). The flow downstream from
the dam is constant (smooth baseline).
Designing a Post-column System
The same principals that are used in HPLC can be applied to the post-column system. What happens if we
simply add a post-column pump, a mixing tee, and a reactor? The periodic noise returns to the baseline
(created by the post-column pump; Figure 1-4).
Pulse
Damper
LC Pump
Injector
Pre-column
Check Valve
Thermostatted
Column & Guard
Detector
W aste
Baseline
FIGURE 1-3
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