Pickering Laboratories Pinnacle PCX - GLYPHOSATE

6.4
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
GLYPHOSATE
6.4 Introduction
6.4-1 Background
6.4-1 Basic Sample Preparation
6.4-2 Reagent Preparation
6.4-4 Analytical and Post-column Conditions
6.4-4 Analytical Procedure
6.4-5 Sample Chromatograms
6.4-6 Precautions
6.4-8 Notes
Introduction
High-performance liquid chromatography (HPLC) with post-column derivatization is a technique for rendering
analytes more detectable than they would otherwise be in their native forms. Post-column derivatization can
give improved sensitivity or better selectivity (reduction of interference) leading to lower detection limits.
The Pickering Laboratories Pinnacle PCX was developed to facilitate the determination of the herbicide
glyphosate (and its metabolite AMPA), meeting or exceeding performance requirements of USEPA Method 547.
The Pickering Post-column method can also be used for the determination of Glyphosate and AMPA in
plants and soils. Pickering has improved sample preparation procedure for vegetable samples. It is a simple
extraction followed by clean-up on a strong cation-exchange cartridge. The procedure is listed later on in this
chapter.