Pickering Laboratories Pinnacle PCX - Sample Chromatograms and Gradient Programs 6

6.2-6
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
COLUMN OVEN PROGRAM
Time Temp ºC
0 34
6 34
17 65
25 70
70 70
71 34
1 Phosphoserine
2 Taurine
3 Phosphoethanolamine
4 Urea
5 Aspartic acid
6 Hydroxyproline
7 Threonine
8 Serine
9 Asparagine
10 Glutamic acid
11 Glutamine
12 Sarcosine
13 -Aminoadipic acid
14 Proline
15 Glycine
16 Alanine
17 Citrulline
18 -Amino-n-butyric acid
19 Valine
20 Cystine
21 Methionine
22 Allo-Isoleucine
23 Cystathionine
24 Isoleucine
25 Leucine
26 Tyrosine
27 Phenylalanine
28 -Alanine
29 -Amino-i-butyric acid
30 Homocystine
31 -Aminobutyric acid
32 Tryptophan
33 Ethanolamine
34 Ammonia
35 Hydroxylysines
36 Ornitine
37 Lysine
38 1-Methylhistidine
39 Histidine
40 3-Methylhistidine
41 Anserine
42 Carnosine
43 Arginine
44 Glucosaminic Acid*
45 2-Aminoethyl-cysteine (AEC)*
HPLC PROGRAM
Time 1700-1125 % Li365 % Li375 % RG003 %
0 100 0 0 0
10 100 0 0 0
19 40 60 0 0
32 0 100 0 0
43 0 100 0 0
43.1 0 0 100 0
57 0 0 100 0
57.1 0 0 70 30
72 0 0 70 30
72.1 100 0 0 0
:
1
2
3
4
5
6
7
8
9
44
45
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
42
41
43
60
min
0 10 20 30 40 50 70
*Internal Standard
NOTE: This method utilizes column temperature gradient. Use Pinnacle PCX column oven or HPLC column oven with temperature
gradient capabilities.
Sample Chromatograms and Gradient Programs
METHOD 1: 0354675T 70-MIN PHYSIOLOGICAL FLUID LITHIUM (4.6 x 75mm)
GUARD COLUMN: GARD COLUMN TEMP: SEE GRADIENT HPLC FLOW RATE: 0.55ml/min
REAGENT FLOW RATE: 0.5ml/min INJECTION VOLUME: 10ul
PEAK IDENTIFICATION