Pickering Laboratories Pinnacle PCX - Page 97

6.2-7
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
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METHOD 2: 0354100T ISOTHERMIC PHYSIOLOGICAL FLUID LITHIUM (4.0 x 100mm)
GUARD COLUMN: GARD COLUMN TEMP: 37°C HPLC FLOW RATE: 0.35ml/min
REAGENT FLOW RATE: 0.3ml/min INJECTION VOLUME: 10ul
PEAK IDENTIFICATION
1. Phosphoserine
2. Taurine
3. Phosphoethanolamine
4. Urea
5. Aspartic acid
6. Hydroxyproline
7. Threonine
8. Serine
9. Aspargine
10. Glutamic acid
11. Glutamine
12. Sarcosine
13. α-Aminoadipic acid
14. Proline
15. Glycine
16. Alanine
17. Citrulline
18. α-Amino-n-butyric acid
19. Valine
20. Cystine
21. Methionine
22. Cystathionine
23. Isoleucine
24. Leucine
25. Norleucine
26. Tyrosine
27. Phenylalanine
28. β-Alanine
29. β-Amino-i –butyric acid
30. Homocystine
31. γ-Aminobutyric acid
32. Tryptophan
33. Ethanolamine
34. Hydroxylysines
35. Ammonia
36. Creatinine
37. Ornithine
38. Lysine
39. Histidine
40. 3-Methylhistidine
41. 1-Methylhistidine
42. Carnosine
43. Anserine
44. α-Amino-β
guanidinopropionic acid
45. Arginie
CONDITIONS
Time (Min) Li275 % Li750 % RG003 % Comment
0 100 0 0 Inject
8 100 0 0 Isocratic
46 65 35 0 Linear Gradient
86 0 100 0 Linear Gradient
90 0 100 0 Isocratic
115 0 94 6 Linear Gradient
122 0 94 6 Isocratic
122.1 100 0 0 Step Change
140 100 0 0 Re-equilibration