Pickering Laboratories Pinnacle PCX - Page 98

6.2-8
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
CONDITIONS
Time (Min) 1700-1125 % Li365 % Li375 % RG003 % Comment
0 100 0 0 0 Inject
15 100 0 0 0 Isocratic
27 40 60 0 0 Linear Gradient
45 0 100 0 0 Linear Gradient
60 0 100 0 0 Isocratic
60.1 0 0 100 0 Step Gradient
78 0 0 100 0 Isocratic
78.1 0 0 70 30 Step Gradient
95 0 0 70 30 Isocratic
95.1 100 0 0 0 Step Gradient
115 100 0 0 0 Equilibration
COLUMN OVEN PROGRAM
Time (Min) Temp. ºC
0 34
13 34
30 65
67 66
80 70
97 70
98 34
METHOD 3: 0354100T TEMP GRADIENT PHYIOLOGICAL FLUID LITHIUM (4.0 x 100mm)
GUARD COLUMN: GARD COLUMN TEMP: SEE GRADIENT HPLC FLOW RATE: 0.4ml/min
REAGENT FLOW RATE: 0.3ml/min INJECTION VOLUME: 10ul
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25
80
min
0 20 40 60
1 Phosphoserine
2 Taurine
3 Phosphoethanolamine
4 Urea
5 Aspartic acid
6 Hydroxyproline
7 Threonine
8 Serine
9 Asparagine
10 Glutamic acid
11 Glutamine
12 Sarcosine
13 a-Aminoadipic acid
14 Proline
15 Glycine
16 Alanine
17 Citrulline
18 α-Amino-n-butyric acid
19 Valine
20 Cystine
21 Methionine
22 Cystathionine
23 Isoleucine
24 Leucine
25 Tyrosine
26 Phenylalanine
27 β-Alanine
28 β-Amino-i-butyric acid
29 Homocystine
30 γ-Aminobutyric acid
31 Tryptophan
32 Ethanolamine
33 Ammonia
34 Hydroxylysines
35 Ornitine
36 Lysine
37 1-Methylhistidine
38 Histidine
39 3-Methylhistidine
40 Anserine
41 Carnosine
42 Arginine
43 Glucosaminic Acid*
*Internal Standard
NOTE: This method utilizes column temperature gradient. Use Pinnacle PCX column oven or HPLC column oven with
temperature gradient capabilities.
PEAK IDENTIFICATION