Pickering Laboratories Pinnacle PCX - Page 99

6.2-9
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
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020406080100 min120140 160180
METHOD 4: 0393250 ISOTHERMIC PHYSIOLOGICAL FLUID LITHIUM (3.0 x 250mm)
GUARD COLUMN: GARD COLUMN TEMP: 40°C HPLC FLOW RATE: 0.30ml/min
REAGENT FLOW RATE: 0.3ml/min INJECTION VOLUME: 10ul
Time(min) %Li275 %Li750 %RG003 Comment
0 100 0 0 inject
17 100 0 0 isocratic
65 65 35 0 linear gradient
128 0 100 0 linear gradient
145 0 100 0 isocratic
185 0 94 6 linear gradient
185.1 100 0 0 step change
210 100 0 0 re-equilibration
PEAK IDENTIFICATION
1. Phosphoserine
2. Taurine
3. Phosphoethanolamine
4. Urea
5. Aspartic acid
6. Hydroxyproline
7. Threonine
8. Serine
9. Aspargine
10. Glutamic acid
11. Glutamine
12. Sarcosine
13. α-Aminoadipic acid
14. Proline
15. Glycine
16. Alanine
17. Citrulline
18. α-Amino-n-butyric acid
19. Valine
20. Cystine
21. Methionine
22. Cystathionine
23. Isoleucine
24. Leucine
25. Norleucine
26. Tyrosine
27. Phenylalanine
28. β-Alanine
29. β-Amino-i –butyric acid
30. Homocystine
31. γ-Aminobutyric acid
32. Tryptophan
33. Ethanolamine
34. Hydroxylysines
35. Ammonia
36. Creatinine
37. Ornithine
38. Lysine
39. Histidine
40. 3-Methylhistidine
41. 1-Methylhistidine
42. Carnosine
43. Anserine
44. α-Amino-β-
guanidinopropionic acid
45. Arginie