6.2-10
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
METHOD 5: 0354100T PKU AND MSUD SCREENING LITHIUM (4.0 x 100mm)
GUARD COLUMN: GARD COLUMN TEMP: 38°C HPLC FLOW RATE: 0.35ml/min
REAGENT FLOW RATE: 0.3ml/min INJECTION VOLUME: 10ul
Time (min) %Li275 %Li750 %RG003 Comment
0 86 14 0 Inject
25 73 27 0 Linear gradient
25.1 0 0 100 Step gradient
30 0 0 100 Isocratic
30.1 86 14 0 Step gradient
42 86 14 0 Re-equilibration
5
10 2015
25 min
Valine
Cystathionine
Methionine
Isoleucine
Leucine
Norleucine
Tyrosine
Phenylalanine
a-Amino-n-Butyric Acid
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