6.2-11
Section 6
ApplicAtions
Pinnacle Operators Manual
Pickering Laboratories Inc.
METHOD 6: 1154110T OXIDIZED HYDROLYSATE SODIUM (4.6X110 MM)
GUARD COLUMN: GARD COLUMN TEMP: SEE GRADIENT HPLC FLOW RATE: 0.6ml/min
REAGENT FLOW RATE: 0.3ml/min INJECTION VOLUME: 10ul
HPLC PROGRAM
Time % Na270 % Na425 % Na640 % RG011
0 100 0 0 0
4.0 100 0 0 0
15.0 0 100 0 0
16.0 0 0 100 0
31.0 0 0 100 0
31.1 0 0 0 100
33.0 0 0 0 100
33.1 100 0 0 0
40 100 0 0 0
COLUMN OVEN PROGRAM
Time Temp ºC
0 55
12 55
17 70
32 70
33 55
1 Aspartic Acid
2 Threonine
3 Serine
4 Glutamic Acid
5 Proline
6 Glycine
7 Alanine
8 Cystine
9 Valine
10 Methionine
11 Isoleucine
12 Leucine
13 Norleucine
14 Tyrosine
15 Phenylalanine
16 Lysine
17 Histidine
18 Ammonia
19 Tryptophan
20 Arginine
21 Cysteic Acid
22 Methionine Sulfone
23 Methionine Sulfoxide
24 Trans-4-Hydroxy-L-Proline
25 D,L & allo-Hydroxylysine
NOTE: This method utilizes column temperature gradient.
Use Pinnacle PCX column oven or HPLC column oven with
temperature gradient capabilities.
PEAK IDENTIFICATION
0
5
10 15 20 25
30
min
21
22
1
2
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