3.11
Section 3
InstallatIon
Pinnacle Operators Manual
Pickering Laboratories Inc.
analytical column. Connect the Column Outlet tubing (pre-formed and cut to the right length at Pickering).
Hang the column in the column oven, use care to ensure the tubing passes through the guide hooks at the top of
the column oven. The guard column should be at the bottom, and the exit of the column at the top.
NOTE: If the application is one that uses sodium or lithium buffers, use deionized water to clean any stainless
steel fittings that have come into contact with buffer to prevent corrosion.
Monitor the pressures and stop the HPLC pump when the pressure stabilizes.
Detector Connections
Connect a 1/16 inch x 0.020" ID tubing from the outlet of the Detector
to the external 100psi back-pressure regulator (PN 3102-9025) using a
1/4-28 nut with a 1/16 inch reversed-ferrule. There is an arrow on the back
pressure regulator indicating direction of flow. Insure that the arrow is
pointing away from the detector and toward the waste line.
Connect the 0.020" ID PTFE tubing provided in the packing kit (PN 2101-
0225) to the outlet of the external 100 psi back-pressure regulator. Place
the other end in an appropriately labeled waste container.
Connect the PEEK union to the outlet of the Ambient reactor (or Heated
reactor if it is a single-pump system) using a Lite-touch Nut and Ferrule.
Connect a 0.011" ID tubing from the outlet of the union at the exit of the
fluidics panel on the right-hand side of the instrument to the inlet of the
detector flow cell.
Set the time constant on the detector to 2–4 seconds.
The pressure rating of the detector flowcell must be >110 psi (7.5 bar). If your detector flowcell is rated lower,
consult Pickering Laboratories.
Special Note to Hewlett-Packard 1046A end-users: Replace the 0.12 mm ID inlet tubing (red) and heat-
exchanger from the left side of the detector to the flowcell (behind the front panel of the detector) with a 0.25
mm ID (blue) tubing (HP Cat. No. 79881-67302) to reduce the back-pressure.
Caution! The 100psi back-pressure regulator is directional. Do not reverse flow!
Figure 3-3
from Detector Exit
Flow direction
to Waste
Eluant Priming
Before proceeding, check for and repair any leaks between the pump and the pre-column check valve. Once
you are certain there are no leaks, do not open the connections between the pump and injector.
IMPORTANT! If any application other than Carbamates is to be used, remove water/methanol and replace
with water. Flush at least 10 ml through lines and flush
injector line. This is to prevent any precipitation issues
between Methanol and the buffers and to prevent any
organic solvents from entering the column. Pickering
Laboratories cation exchange columns for Amino Acids
and Glyphosate analysis will be damaged by organic
solvents.
Fill the reservoirs with the appropriate eluants/mobile
phases and again flush at least 25 ml OF EACH ELUANT
with the purge valve in the open position.
Close the purge valve, and pump each eluant at 2 ml/
min for 5 minutes.
If buffers will be used as the eluant, use pH paper to
measure the pH of the solution coming through the
tubing.
Column and Guard Installation
Set the HPLC to 100% of the storage solution in the
column.
Install the pre-column check valve and female nut
inline before the column over door. Reference the
Flow Diagram in section 8.5 for complete parts list of
the pre-column check valve.
Start the flow rate to 0.2 ml/min.
Connect the line from the outlet of the pre-column
check valve to the inlet of the guard column.
When liquid exits the guard column, connect the
“column connector/coupler” provided in the packing
kit. When liquid exits the connector, connect the
Figure 3-2
Column outlet tubing
Column oven door
Rubber foam seal
Analytical column
Inlet end
from HPLC
Check Valve
(3106-1007)
PEEK Nut
(1452-0226)
PEEK Nut
(3101-0011)
Blue Peek Tubing
to Guard
Column
Coupler/column connector
Guard column
Check valve
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