3.3
Section 3
InstallatIon
Pinnacle Operators Manual
Pickering Laboratories Inc.
Note: If TRIONE
®
is to be used for Reagent 1, Nitrogen must be used to prevent out-gassing.
A waste container should be provided for the waste lines from the Pinnacle PCX and the HPLC detector.
HPLC SYSTEM REQUIREMENTS
Since every HPLC is different, the following procedure has been generalized. Before attempting to connect any
tubing, examine the HPLC setup, and determine the best possible means of making the connections. Small ID
tubing (0.011") should be used wherever the sample is in the flow path.
Important! If the system will be used for amino acids, glufosinate, glyphosate, polyamines, or diquat &
paraquat analysis, be aware that the column regenerant is strongly alkaline. Any polymers or other materials in
the HPLC pump, injector, needle seat, and detector must be compatible. For example, the standard rotor seal in
Rheodyne injector valves is Vespel® polyimide, which is not recommended at pH >9; a Tefzel® or PEEK rotor
seal must be installed.
For all applications, the pressure rating of the detector flow cell must be > 110 psi (7.5 bar)
FOR AMINO ACID ANALYSIS
Pump
Minimum ternary gradient elution
Piston wash capability is preferable
Injector
Tefzel or PEEK rotor seal for injector valve
Tefzel or PEEK needle seat if it is an autosampler
FOR GLYPHOSATE ANALYSIS
Pump
Minimum binary gradient elution
Piston wash capability is preferable
Injector
Tefzel or PEEK rotor seal for injector valve
Tefzel or PEEK needle seat if it is an autosampler
For water samples, at least 200 μl injection
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