4.16
Section 4
Pinnacle PcX OPeratiOn
Pinnacle Operators Manual
Pickering Laboratories Inc.
NOTE: after starting a new sequence always make sure that first injection signal was received normally by
checking that Pinnacle PCX timer matches elapsed time of the HPLC run. Pinnacle PCX will stop the sequence if
no injection signal is received from HPLC in 15 min from selecting Start Sequence.
After the post-column method Run time is done Pinnacle PCX turns the pump(s) OFF , checks temperature and
flow rate settings for the next run and automatically refills the reagent. Pinnacle PCX will turn the pump(s) ON
5 min before the end of the Equilibration time.
Once next injection signal is received Pinnacle PCX starts the second method in the sequence. The number
of the current run and total number of runs in the Sequence are shown on the bottom of the Pinnacle PCX
software.
After Sequence is complete Pinnacle PCX turns OFF the pump(s). Message “Sequence done. (Runs n of N)” is
displayed in the software window.
Important Practical Considerations
Always match number of runs in HPLC and Pinnacle PCX sequences. If more samples are added to HPLC
sequence or samples are removed edit Pinnacle PCX sequence accordingly. Total number of samples displayed
by HPLC software often does not include calibrators and controls. Make sure you count total number of runs in
HPLC sequence not just number of samples before creating Pinnacle PCX Sequence.
To edit the running Sequence:
- select Sequence – Edit/Delete
- select the Sequence name from the Sequence Box.
- change number of runs of the currently running Method or add a new line to the Sequence anywhere below
the Method in progress.
- select Save and then Load from the Actions tabs.
- check number of runs on the bottom of the main software window to confirm that changes has taken affect.
We recommend restarting Pinnacle PCX software after Sequence is completed.
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