4.18
Section 4
Pinnacle PcX OPeratiOn
Pinnacle Operators Manual
Pickering Laboratories Inc.
AUTOMATIC SHUTDOWN
Create a Shutdown Method for Pinnacle PCX using the following settings as an example:
Run time: 30 min
Equilibration time: 5 min
Reactor temperature: 35 C
Column temperature: 35 C
Pump(s) flow rate: 0 mL/min
Set it up as the last method in the Pinnacle PCX sequence. Make
sure pump flow rate is set to 0 mL/min and reactor temperature
is set close to room temperature. You can leave your column at
your operating temperature or set it at room temperature.
Create corresponding slowdown Method for HPLC. Set it up as
the last method in the HPLC sequence. Make sure HPLC flushes
column and reactor with column storage eluant for at least 30 min.
RECOMMENDED ACCOMPANYING HPLC SLOWDOWN METHOD
Set the HPLC to 100% Storage Eluant (see application section for proper eluant for your column), and set the
HPLC pump at the normal analytical flow rate. Choose an eluant that elutes contaminants from the column; for
example, methanol for a reversed-phase column and regenerant for an ion-exchange column.
Time (min) % Storage Eluant Flow (mL/min)
0 100 Analytical flow rate*
30 100 Analytical flow rate*
30.1 100 0.0
*Follow instructions that come with the column.
Figure 4.7
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