Home
revvity
Cash Counter
CELLOMETER ASCEND
revvity CELLOMETER ASCEND - User Manual
61 pages
Manual
Specs
Ask a question
Save Page as PDF
To Next Page
To Next Page
Loading...
US
ER
MA
NUAL
CELLOMETER
™
ASCEND
FLUORESCENT
CELL COUNTER
8004708
Rev
A
For Research Use Only. Not for use in
diagnostic p
rocedures
.
2
Table of Contents
Main Page
default chapter
3
Table of Contents
3
Chapter 1. Introduction
7
Cellometer Ascend Instrument Overview
7
Intended Use
7
Contents of Shipping Container
8
Matrix Software Overview
8
Matrix 21 CFR Part 11 Module Overview
9
About this User Manual
10
Glossary of Abbreviations
11
Glossary of Symbols
12
Chapter 2. Hazards, Safety, and Environment
13
Potential Hazards
13
Electrical Hazard
13
Instrument System Hazard
14
Servicing Hazard
14
Safety Information
15
Safety Protocols
15
Safety Features
15
Federal Communications Commission
15
Environmental Requirements
16
Chapter 3. Instrument Description
17
Ascend Components
17
Device SN Label
17
Instrument Specifications
18
Technical Information
19
Declaration of Conformity
19
Chapter 4. Unpacking and Site Preparation
21
Unpacking and Setting up Instrument
21
Site Preparation
22
Facility Requirements
22
Transporting the Instrument
22
Disposal of Waste Electrical and Electronic Equipment
22
Instrument and Software Validation
23
Cellometer Ascend IQ/OQ Validation
23
21 CFR Part 11 OQ Validation
23
Chapter 5. Operation
25
Launching the Software
25
Matrix Screen Elements
25
Navigation Bar
26
Workflow Tabs
26
Simplified Workflow
28
User Favorites
28
Streamlined User Experience
28
Focus Methods
29
Image-Based Auto Focus
29
Slide-Based Auto Focus
29
Viewing Software Version
30
Chapter 6. Viability Methods
31
Evaluating Viability Methods
31
Using Trypan Blue Viability Method
31
Preparing a Cell Sample for Trypan Blue Viability Determination
31
Trypan Blue Staining Solution Guidelines
31
Using AO/PI Viability Method
32
Preparing a Cell Sample for AO/PI Viability Determination
32
AO/PI Staining Solution Guidelines
33
Chapter 7. Sample Preparation
35
Preparing Counting Chamber Slides
35
Loading Samples into Counting Chambers
35
Chapter 8. Matrix Counting and Analysis Workflow
37
Performing a Count
37
Choosing Data Acquisition Workflow
37
Selecting a Favorite
37
Entering Parameter Settings
38
Loading Samples
39
Preview Mode
40
Previewing Live Images
40
Adjusting Focus
40
Previewing Channel Images
41
Count Mode
42
Analyzing Scan Results
43
Understanding Default Report Tabs
43
Changing Well View Image Display
44
Varying Well View Channels/Counted Overlay Display
44
Understanding Custom Reporting
44
Exporting/Printing Scan Result Data
45
Verifying Auto Export
45
Exporting and Printing Scan Results (Manual Settings)
46
Performing a Recount
47
Refining Assay Details/Selecting New Assay
47
Managing Channel Mappings
47
Modifying Auto Export Options
48
Tapping the Recount Button
48
Best Practices and Workflow Tips
49
Chapter 9. Cleaning, Maintenance and Storage
51
Cleaning
51
Routine Maintenance
51
Contacting Support
51
Storage
52
Chapter 10. Troubleshooting and Faqs
53
Troubleshooting and Instrument Messages
53
Frequently Asked Questions
54
Chapter 11. Revvity Support
55
Scope of Support Services
55
Contact Methods
55
Reporting an Issue to Support
55
Generating Diagnostic Reports
56
Appendix A. Consumables
57
Counting Chamber Slides
57
Assay Reagents and Kits
57
Appendix B. Warranty and License Details
59
Warranty Information
59
Terms and Conditions
59
Limitation of Liability (Hardware and Software)
60
Need help?
Do you have a question about the revvity CELLOMETER ASCEND and is the answer not in the manual?
Ask a question
revvity CELLOMETER ASCEND Specifications
General
Brand
revvity
Model
CELLOMETER ASCEND
Category
Cash Counter
Language
English
Need help?
General
To Next Page