revvity CELLOMETER ASCEND - AO;PI Staining Solution Guidelines

Chapter 6. Viability Methods
8004708 Cellometer™ Ascend User Manual Rev A 27
The table below shows the recommended dilution when preparing cell samples for AO/PI viability analysis and the
final Dilution Factor to enter into the Matrix software for a variety of sample types.
Sample Type
Preliminary Sample
Dilution
Volume of
Sample
Volume of
AO/PI
Final Dilution
Factor
Whole peripheral blood or
cord blood
1:10
20 μL
20 μL
20
PBMCs following Ficoll separation
Not Required
20 μL
20 μL
2
Mononuclear cells from
processed bone marrow
Not Required
20 μL
20 μL
2
Tumor digest/Tissue digest
Not Required
20 μL
20 μL
2
Stem cells from CD34+ separation
Not Required
20 μL
20 μL
2
AO/PI STAINING SOLUTION GUIDELINES
Use the following AO/PI staining solution guidelines when preparing cell samples for analysis.
Stain Type
Use with Cell Sample
Dilution Factor
AO (CS1-0108-5mL)
1:1
2
PI (CS1-0109-5mL)
1:1
2
AO/PI (CS2-0106-5mL)
AO/PI (CS2-0106-25mL)
1:1
1:1
2
2