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revvity CELLOMETER ASCEND - Frequently Asked Questions

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Chapter 10. Troubleshooting and FAQs
8004708 CellometerAscend User Manual Rev A 48
FREQUENTLY ASKED QUESTIONS
How do I order a replacement for an instrument Power Cord that has been lost?
Contact Support with the serial number of the instrument to request a replacement power cord/cable.
When testing for cell viability, why are my count results lower than expected?
When preparing a sample to test for cell viability, ensure that the stock solution is being used as indicated in
Trypan Blue Staining Solution Guidelines on page 25 or AO/PI Staining Solution Guidelines on page 27. Using stains
in a concentration higher than recommended concentrations will make the cells more difficult to detect and may
result in counting inaccuracies.
In addition, ensure that:
The viability method is appropriate for the cell type. See Evaluating Viability Methods on page 25 for details.
The current assay and/or cell type is appropriate for the sample. See the Cellometer Matrix Software User
Manual for more information about editing assays and cell type parameter settings.

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