Chapter 5. Operation
8004708 Cellometer™ Ascend User Manual Rev A 23
Single Folder Button: When entering setup details in the Reports and Exports
area, the Single Folder button toggles between exporting files using the hierarchical
folder structure traditionally available in earlier Matrix releases (button is not
selected) or to a single folder (i.e., button is selected). This button is also available
when exporting scan results and is disabled by default.
Back Button: While using the Acquire workflow tab, the Matrix software launches
a series of screens that guide you through a sequential process. Use the Back button
located in bottom left corner of the screen to return to the previous screen in the
process. Tapping the Acquire tab while already in the workflow no longer returns to the
previous screen as it did in earlier releases.
Select Result Button: While using the Data workflow tab, the Matrix software
launches a series of screens that guide you through a sequential process. Use the
Select Result button located in bottom left corner of the screen to return to the
previous screen in the process. Tapping the Data tab while already in the workflow
no longer returns to the previous screen as it did in earlier releases.
Focus Methods
Matrix software offers the following two focus methods for the Cellometer Ascend.
IMAGE-BASED AUTO FOCUS
The image-based auto focusing operation relies on the contrast within an image. Z-stacking is performed on the
initial image, then the software analyzes objects in the image, selects images with the best contrast, and applies an
algorithm to sharpen the focus further. Users have the option to perform image-based auto focusing for either the
brightfield or fluorescent channels.
SLIDE-BASED AUTO FOCUS
Available for the Cellometer Ascend for the 3-Chamber Slide Only. The Slide-based auto focusing operation allows
the software/instrument to focus on the consumable (without needing to focus directly on the sample) by using a
target manufactured on the slide.
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