Chapter 8. Matrix Counting and Analysis Workflow
8004708 Cellometer™ Ascend User Manual Rev A 43
BEST PRACTICES AND WORKFLOW TIPS
• The Images Per Well field in the Setup screen allows users to specify the number of images to be taken of the
counting chamber/well. Choosing the lowest number of images (e.g., 2) provides a high-level overview of data
and provides the fastest counting speed. Choosing 4 or 8 images increases the amount of data collected by
partitioning the sample into sections and averaging the results from all images, thus improving the precision of
calculations for statistical analysis but requiring more time to obtain count results. Regardless of the number
of Images Per Well selected, count results are for the entire counting chamber or “well” (i.e., representing an
average of the results).
• While using the Acquire workflow tab, the Matrix software launches a series of screens that guide you through
a sequential process. Use the Back button located in bottom left corner of the screen to return to the
previous screen in the process. Tapping the Acquire tab while in the workflow no longer returns to the previous
screen as it did in earlier releases.
• While using the Data workflow tab, use the Select Result button to review other count results after
viewing the currently selected scan. Note that when a scan result is created for a sample as the result of a
count, it is displayed at the top of the Results List. Double-tap on any scan result in this list to view it. See the
Cellometer Matrix Software User Manual for details on managing scan results contained in the Results List.
Tapping the Data tab while in the workflow no longer returns to the previous screen as it did in earlier releases.
• When viewing scan results, if you find it necessary to fine-tune assay and/or cell type parameter settings and
re-analyze the data, tap the Recount button at the bottom of the Results screen. You can edit either Last Used
Assay parameters or select a new Assay for Recount and edit its parameters as necessary to meet your
requirements. See the Cellometer Matrix Software User Manual for details on editing assay and/or cell type
parameter settings.
Note: If you need help optimizing assay and/or cell type parameter settings, contact Support by visiting
https://www.revvity.com/contact-us or send an email to: CellC-support@revvity.com
• When exporting scan results, the Matrix software creates a hierarchical folder structure (by default) in the
export location defined for the scan result – a top-level consumable_ID folder (where consumable_ID
represents the name you entered in the Setup screen) and a <date_time> subfolder (where date_time
represents the date/time stamp of scan images). Within this second-level folder are additional folders (i.e., a
consumable_ID folder containing scan images and a <date_time> folder containing the initial count results).
Contents of this <date_time> subfolder will vary based on selected export options. Each time you perform a
recount on the scan images, a new <date_time> subfolder is created for the results.
When entering setup details in the Reports and Exports area, users can select the Single Folder button
which toggles between exporting files using the hierarchical folder structure described above
(button is not selected) or to a single folder (i.e., button is selected). This button is also available when
exporting scan results. This button is disabled by default.
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