revvity CELLOMETER ASCEND - Instrument Specifications

Chapter 3. Instrument Description
8004708 Cellometer™ Ascend User Manual Rev A 12
INSTRUMENT SPECIFICATIONS
The Cellometer Ascend is your all-in-one automated cell viability counter. Using both Brightfield and Dual-
Fluorescent imaging in combination with
pattern-recognition software, it quickly and accurately identifies and
counts cells.
For cultured cells stained with trypan blue or fluorescent reagents, the Ascend simultaneously
calculates
Live/Dead and Total cell counts, Viability (%), and Live and Total cell concentrations, including a helpful
graphic overlay used to highlight live and dead cells with color-coded outlines in the image being viewed.
Cellometer Ascend
Channels Brightfield and Fluorescent (FL1 - Green and FL2 - Red)
Fluorescent Optics
Excitation / Emission:
470 nm / 534 nm
Example Fluorophores:
- Acridine Orange (AO)
- Calcein AM
- CFDA
- GFP (Green Fluorescent Protein)
- SYTO™ 9 and SYTO™ 13
Excitation / Emission:
531 nm / 655 nm
Example Fluorophores:
- Propidium Iodide (PI)
- Ethidium Bromide (EtBr)
Commonly Used
Compatible Dyes
AO/PI, Trypan Blue
Counting Speed (Per Sample)
<30 sec
Volume (Per Chamber)
3-Chamber Slide: 20 µL
8-Chamber Slide: 10 µL
Cell Size/Diameter Range
4 – 90 µm
Concentration Range
1x10
5
– 1x10
7
cells/mL
Weight
24 lbs (10.5 kg)
Width
9.9″ (25 cm)
Depth
13″ (32.8 cm)
Height
18.7″ (47.5 cm)
Input to Power Adapter 100-240 VAC, 50/60 Hz, 1.5 A
Output to Instrument 12 VDC, 10 A
The Cellometer Ascend uses brightfield and fluorescent imaging with pattern-recognition software to quickly and
accurately decluster, identify and count individual cells.