Table of Contents
8004708 Cellometer™ Ascend User Manual Rev A iv
CHAPTER 5. OPERATION ........................................................................................................ 19
Launching the Software .......................................................................................................................... 19
Matrix Screen Elements .......................................................................................................................... 19
Navigation Bar ................................................................................................................................................... 20
Workflow Tabs ................................................................................................................................................... 20
Simplified Workflow .......................................................................................................................................... 22
User Favorites................................................................................................................................................................. 22
Streamlined User Experience ......................................................................................................................................... 22
Focus Methods .................................................................................................................................................. 23
Image-Based Auto Focus ................................................................................................................................................ 23
Slide-based Auto Focus .................................................................................................................................................. 23
Viewing Software Version ...................................................................................................................... 24
CHAPTER 6. VIABILITY METHODS ........................................................................................... 25
Evaluating Viability Methods .................................................................................................................. 25
Using Trypan Blue Viability Method .................................................................................................................. 25
Preparing a Cell Sample for Trypan Blue Viability Determination .................................................................................. 25
Trypan Blue Staining Solution Guidelines ....................................................................................................................... 25
Using AO/PI Viability Method ............................................................................................................................ 26
Preparing a Cell Sample for AO/PI Viability Determination ............................................................................................ 26
AO/PI Staining Solution Guidelines ................................................................................................................................ 27
CHAPTER 7. SAMPLE PREPARATION ...................................................................................... 29
Preparing Counting Chamber Slides ....................................................................................................... 29
Loading Samples into Counting Chambers ............................................................................................. 29
CHAPTER 8. MATRIX COUNTING AND ANALYSIS WORKFLOW ................................................ 31
Performing a Count ................................................................................................................................ 31
Choosing Data Acquisition Workflow ................................................................................................................ 31
Selecting a Favorite ........................................................................................................................................................ 31
Entering Parameter Settings .......................................................................................................................................... 32
Loading Samples ................................................................................................................................................ 33
Preview Mode .................................................................................................................................................... 34
Previewing Live Images .................................................................................................................................................. 34
Adjusting Focus .............................................................................................................................................................. 34
Previewing Channel Images ............................................................................................................................................ 35
Count Mode ....................................................................................................................................................... 36
Analyzing Scan Results ............................................................................................................................ 37
Understanding Default Report Tabs .................................................................................................................. 37
Changing Well View Image Display .................................................................................................................... 38
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