174 ADVIA 1800 Operator’s Guide
Priming and initializing the ISE module
1. At the ISE Operation window, in the Bufferprime area, enter 15 in the Times field.
2. To prime the line with buffer, select
Execute, then select Yes.
3. When the priming is finished, verify that the electrodes are not leaking.
4. At the ISE Operation window, select
Exit, then select Yes.
5. Run 10 pooled serum samples, or do an ISE CV check.
6. Perform calibration and run controls.
Replacing ISE electrodes
Materials required:
Electrodes
• Cl (REF 07097504, PN 073-0049-01)
• K (REF 06135445, PN 073-0050-01)
• Na (REF 03092699, PN 073-0051-01)
• Reference (REF 00311764, PN 073-0653-01
• O-rings, 3 (REF 09955206, PN 073-0071-01)
• Philips screwdriver
Time: 5 minutes
Analyzer mode: Manual operation
BIOHAZARD
Wear personal protective equipment.
Use universal precautions.
Replace the Na, K, and the Cl electrodes if the slope is incorrect or calibration
continuously fails.
The acceptable ISE slope is between 45.0 and
63.0. Slopes outside of this range are flagged
as shown in the table. A flagged slope fails
the calibration. The slope limits are defined at
the ISE Parameter Settings window.
Mark ISE Slope Range
H > 65.0
h 63.1 to 65.0
l 38.0 - 44.9
L < 38.0
Replace the reference electrode when the reference electrode value is <500.
Checking the reference electrode value
1. At the Menu Panel, select
Maint., then select ISE Monitor.
2. At the ISE Monitor window, at the bottom of the Calib.monitor: Serum area, check
the value of the Ref. electrode field.
3. If the Ref. electrode value is
less than 500.0, replace the reference electrode.
Removing electrodes
1. At the Menu Panel, select Maint., then select ISE Operation.
2. In the Period. wash area, select
OFF, then select Set.
3. Using a Phillips screwdriver, remove the screws (
1, see Figure 5-2) that secure the
DPP shield to the analyzer panel.
To Next Page
Loading...
