Troubleshooting 177
Conditioning the Na and K electrodes
Materials required:
•
10 mL of serum pool
•
30 mL of ISE Buffer
(REF 03463190, PN B01-4171-51)
•
2-mL or 3-mL plastic, disposable
pipette
Time: 5 minutes (preparation)
24 hours (immersion)
BIOHAZARD
Wear personal protective
equipment.
Use universal precautions.
NOTES
• The Cl electrode does not require conditioning.
• If the slope of the electrode is in the range of 46 – 49 (Na or K) and the Daily
Maintenance Log entries for the electrode shows it is trending down, then perform
this procedure.
• If the slope is low and a trend is not observed, verify that all other ISE maintenance is
current before performing this procedure.
• Condition an electrode the day before you need to use it. A new electrode may take a
long time to stabilize.
1. Prepare a 1:4 dilution of pool serum using ISE buffer solution.
2. Remove the new electrode from its case.
NOTE: The ion electrode contains an inner solution, which can be confirmed by
shaking the electrode. This solution decreases little by little with time. If you do not
feel any response in your shaking, measure its weight. If the electrode weighs less
than 9 g, do not use it.
3. Remove the sponge from the bottom of the
electrode case and place the electrode to be
conditioned back into the case.
4. U
sing a dropper or pipette, add 0.5 mL of
pool serum drop by drop into the flow path
of the electrode.
5. Be sure to apply the serum thoroughly.
6. Cover the entire electrode with ISE buffer.
Allow the electrode to condition overnight.
NOTE: Be sure that the electrode is at room
temperature. There is a lot of drift if the
electrode is not at room temperature.
7. When conditioning is complete, remove the
electrode, wash it with deionized water, and
Figure 5-38. Soaking the electrode
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