INSTRUMENT DESCRIPTION
Cell Observer SD Suitability of Objectives for the Use Carl Zeiss
M60-2-0021 e 01/2009 423638-7044-001 31
2.5 Suitability of Objectives for the Use
The functional principle described in section
2.2 imposes certain requirements for selection of objectives.
The pinhole diameter is 50 µm, a pair of neighbouring pinholes are located at a distance of 250 µm from
each other. The pinhole diameter is optimized for strongly magnifying objective lenses with a high
numerical aperture (100x / 1.4 or similar).
Using the CSU-X1 with weakly magnifying objectives will result in specimen sections with a thickness by
far greater than 1 AU (Airy Unit).
2.6 Suitability of Optical Fibers for Use in Cell Observer SD
For this application, the optical fiber cable is factory-shipped as an integral part of the spinning disc unit.
The total laser power which is injected into the fiber cable must not exceed a value of 100 mW!
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