Art: 714376-00M Rev. Date: 31-Jan-12 14-7
Before Use
Equilibrate the ampule for approximately 30 minutes at room (ambient)
temperature.
Procedure
STEP ACTION
1 Access the i-STAT Cartridge Control option under Quality Tests in
theAdministrationMenu.Entertherequiredinformation.The
analyzerallows15minutes(orthecustomizedtimeout)toinsert
the cartridge after the last data entry.
2 Immediatelybeforeuse,shaketheampulevigorouslyfor5to10secondsto
equilibrate the liquid and gas phases.
Toshake,holdtheampuleatthetipandbottomwithforenger
and thumb to minimize increasing the temperature of the solution.
Ifnecessary,tapthetipoftheampuletosendsolutionbackinto
the bottom section of the ampule.
3 Protectngerswithgauze,tissueorglove,oruseanampulebreaker
to snap off the tip of the ampule at the neck.
4 Immediately transfer the solution from the ampule into a capillary
tubeorsyringe,andthenimmediatelytransferthesolutionintoa
cartridge.
5 Immediately seal the cartridge and insert it into an analyzer – it is
importantnottoexposethesolutiontoroomairsincethiswillalter
theresults.Note:Sinceaqueousbasedsolutionssuchascontrols
lackthebufferingcapabilitiesofwholeblood,thetransferprocess
from ampule to cartridge must be more expedient than with a
patient sample.
Transfer with Capillary
Tube
Plain capillary tubes are recommended to transfer an aqueous control from the
ampuletothecartridge.Whenusingacapillarytube(freshcapillarytubeswith
sufcientllcapacityarerecommended),llfromthebottomoftheampule
toavoiddrawingairintothecapillarytube.Avoiddrawingsolutionfromthe
surface by placing a finger over the far end of the tube as it is inserted into the
ampule.Oncetheopenendofthetuberestsatthebottomoftheampule,
uncovertheotherendtoallowllingbycapillaryaction.
Transfer with Syringe
Plain syringes are recommended to transfer an aqueous control from the ampule
tothecartridge.Whenusingasyringe(fresh1ccor3ccsterilesyringewith
16-20gaugeneedlesarerecommended),slowlydrawapproximately1mLof
solution from the bottom of the ampule.
Ifairistrappedbetweentheleadingedgeofthesolutionandtheplunger,do
notinvertthesyringetoexpelit;thiswillnotaffectsolutionnearthetipof
the syringe.
Ifairbubblesarecontinuallydrawnintothesyringe,orifabubbleistrapped
nearthe tipofthe syringe,discardthe ampuleandsyringe andusea fresh
ampule and syringe.
Expeloneortwodropsfromthesyringebeforellingthecartridge.
Target Values
Target values (determined by testing multiple ampules of each level using
multiplelotsofcartridgesandi-STATanalyzersthathavepassedtheElectronic
Simulatortest)areprintedonavalueassignmentsheetpostedontheAPOC
websiteatwww.abbottpointofcare.com.
Alwaysbesurethatthelotnumberprintedontheinsertmatchesthelotnumber
onthelabeloftheampuleinuse,andthatthesoftwarerevisionabovethe
targetvaluetablematchesthesoftwarerevisionintheanalyzer.
To Next Page
Loading...
Need help?
General
