Agilent 1260 Infinity DAD and MWD User Manual 151
Test Functions
8
Holmium Oxide Test
Holmium Oxide Test
The holmium oxide test uses characteristic absorbance maxima of the built-in
holmium oxide filter to verify wavelength accuracy (see also “Wavelength
Verification and Calibration” on page 160). When the test is started, the 1-nm
slit is moved into the light path automatically. To eliminate effects due to
absorbing solvents, the test should be done with water in the flow cell or with
removed flow cell.
Test Evaluation
Holmium Oxide Test Evaluation
Limits:
The test is evaluated by the instrument, and the measured maxima are
displayed automatically. The test fails if one or more of the maxima lies
outside of the limits (see Figure 51 on page 152).
See also “Declaration of Conformity for HOX2 Filter” on page 301.
361.0 nm 360.0 - 362.0 nm (± 1nm)
418.9 nm 417.9 - 419.9 nm (± 1nm) (not with ChemStation)
453.7 nm 452.7 - 454.7 nm (± 1nm)
536.7 nm 535.7 - 537.7 nm (± 1nm)