Applied Biosystems 7500 User Manual

Chapter 6 Performing the Pure Dye Calibration
Analyzing the Pure Dye Calibration Data
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 77
Notes
6
Analyzing the Pure Dye Calibration Data
When you run each pure dye plate, the SDS Software
automatically creates and saves a plate document for
each dye. After you calibrate all of the pure dye
plates, the plate documents remain open behind the
plate document displayed by the software. To
complete the calibration, analyze all open pure dye
documents as explained below.
1. From the Windows menu, select the plate
document to analyze.
2. Select the Results tab, then select the Spectra
tab.
3. Select all wells of the plate document by clicking
the upper-left corner of the plate grid.
4. Click (or select AnalysisExtract Pure
Spectra).
The SDS software completes the extraction, then
displays a message (see next page).
The SDS Software creates a
plate document for each
calibrated dye (behind the
visible plate document)
Select
Select
Click
Click

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)