Applied Biosystems 7500 User Manual

Appendix A
Decontaminating the Sample Block
108 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Decontaminating the Sample Block
PHYSICAL INJURY HAZARD. Do not remove the instrument
cover. There are no components inside the 7300/7500/7500 Fast system that you can
safely service yourself. If you suspect a problem, contact an Applied Biosystems Service
Representative.
PHYSICAL INJURY HAZARD. During instrument operation, the
sample block can be heated to 100 °C. Before performing the following procedure, be
sure to wait until the sample block reaches room temperature.
The following procedure explains how to eliminate fluorescent contaminants from the
sample block of the 7300/7500/7500 Fast instrument. Perform the procedure to resolve
problematic background runs where one or more wells consistently exhibit abnormally
high signals, indicating the presence of a fluorescent contaminant.
Time Required 30 minutes
Materials
Required
Cotton or nylon
swabs and lint-
free cloths
10% bleach
solution
Pipette (100-µL)
with pipette tips
Powder-free
gloves
95% ethanol
solution
Deionized
water
Safety
glasses
Screwdriver

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)