Applied Biosystems 7500 User Manual

Chapter 6 Performing the Pure Dye Calibration
Evaluating Pure Dye Calibration Spectra
80 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Evaluating Pure Dye Calibration Spectra
Use the tables in this section to verify that the peak for the spectra of the pure dye occurs
at the correct filter for your system:
• 7300 system – see page 80
• 7500 system – see page 82
• 7500 Fast system – see page 84
If the peak for the spectra of the dye occurs in the wrong filter, you may have run the
wrong dye plate during the calibration. Repeat the procedures for only that dye.
Table 1 Spectra for pure dyes supported by the 7300 system
Instrument Pure Dye Filter Peak (nm)
7300 System FAM A ~520 nm
JOE B ~550 nm
NED C ~550 nm

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)