Applied Biosystems 7500 User Manual

Chapter 6 Performing the Pure Dye Calibration
Preparing for the Calibration
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 71
Notes
6
Preparing for the Calibration
IMPORTANT! Before performing a pure dye
calibration, you must perform an ROI calibration (see
page 35), a background calibration (see page 49), and
an optical calibration (7500 systems only, see
page 59).
IMPORTANT! Wear powder-free
gloves when you handle the pure dye
plates.
1. Retrieve the spectral calibration kit from the
freezer, then remove all of the pure dye plates.
2. Return the spectral calibration kit to the freezer.
3. Allow the pure dye plates to warm to room
temperature (approximately 5 minutes).
IMPORTANT! Do not remove a pure dye plate
from its packaging until you are ready to run it.
The fluorescent dye in the wells of each pure dye
plate is photosensitive. Prolonged exposure to
light can diminish the fluorescence signal
strength of the plate.
Continue with “Creating a Plate Document for the
Pure Dye Calibration” on page 72.
Spectral calibration kit
FAM pure dye plate
>00:05:00Ambient

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)