Applied Biosystems 7500 User Manual

Chapter 4 Performing the Regions of Interest (ROI) Calibration
Overview
36 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Overview
Note: You can also use the Real-Time System Setup wizard to perform the procedure in
this chapter. To access the wizard, select Program Files 7300/7500/7500
Fast System Real-Time System Setup Wizard.
Time Required 30 minutes
Materials
Required
When to Perform
the ROI
Calibration
Perform a regions of interest (ROI) calibration:
• When installing the system (you must perform all calibrations in this order: ROI
backgroundoptical (7500/7500 Fast systems only)pure dyeinstrument
verification).
• Every 6 months, or as often as necessary, depending on instrument use.
• After replacing the lamp.
IMPORTANT! After every ROI calibration, you must perform a background calibration,
optical calibration (7500/7500 Fast systems only), pure dye calibration, and instrument
verification.
GR2170
Powder-free
Gloves
Safety
Goggles
ROI Calibration Plate
Centrifuge with plate
adapter
Applied Biosystems
Real-Time PCR System
Spectral Calibration Kit

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)