Applied Biosystems 7500 User Manual

Chapter 6 Performing the Pure Dye Calibration
Creating a Plate Document for the Pure Dye Calibration
72 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Creating a Plate Document for the Pure Dye Calibration
1. In the SDS software, click (or
select FileNew).
2. Configure the New Document dialog box:
a. Select AssayPure Spectra.
b. Select Container96-Well Clear.
c. Select Temp l a teBlank Document.
d. In the Operator field, enter your name.
e. In the Comments field, enter any
information that you want to attach to the
file (such as the plate bar code).
f. Click .
Note: It is not necessary to name or save
the pure dye plate document. The SDS
software automatically saves the pure dye
data to a calibration file on the computer
hard drive.
The Pure Spectra Calibration Manager is
displayed.
Continue with “Preparing Plates and Performing the
Pure Dye Calibration” on page 73.
a
b
c
d
e
f

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)