Applied Biosystems 7500 User Manual

Appendix C
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 127
Notes
C
Creating a Custom Pure Dye Plate
Overview
The Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System can be used to
run assays designed with custom dyes (dyes not manufactured by Applied Biosystems).
Custom dyes must fluoresce within the spectral range measured by the instrument:
• 500 to 650 nm for 7300 systems
• 500 to 700 nm for the 7500/7500 Fast systems
Before using custom dyes with the 7300/7500/7500 Fast instrument, you must:
• Determine optimum dye concentration
• Create a custom pure dye plate
• Add the custom dye to the software
• Perform a pure dye calibration (see Chapter 6, “Performing the Pure Dye
Calibration.”
Materials
Required
Optical Adhesive Cover
or Optical Flat Caps
ABI PRISM
®
Optical
96-Well Reaction Plate
and Optical 96-Well Fast
Plate
Custom dye(s)
Powder-free
gloves
Safety
glasses
Tubes
(2-mL and 10-mL)
Pipettors and pipette tips
(200-µL and 1000-µL) Water,
deionized
GR2170
Centrifuge with plate
adapter

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)