Applied Biosystems 7500 User Manual

Chapter 7 Verifying the Instrument Performance
Creating a Plate Document for the RNase P Verification Run
92 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Creating a Plate Document for the RNase P Verification Run
1. In the SDS software, click (or
select FileNew).
2. In the New Document dialog box:
a. Select AssayAbsolute Quantification
(Standard Curve).
b. Select Container96-Well Clear.
c. Select Temp l a teAQ RNase P Install.
d. In the Operator field, enter your name.
e. In the Comments field, enter any
information that you want to attach to the
file (such as the plate bar code).
f. In the Default Plate Name field, enter the
bar code of the RNase P plate.
g. Click .
3. In the SDS software, select FileSave As.
IMPORTANT! Do not modify the plate
document. The sample, detector, and method for
the run are coded into the software.
4. Save the document. In the Save As dialog box,
select the folder:
If the Save in field does not display SDS
Documents, navigate to D: driveApplied
BiosystemsSDS Documents.
After selecting the folder, Click .
Prepare and run the RNase P plate as explained
on page 93.
a
b
c
d
e
g
f
Select
Should be
SDS Documents

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)