Applied Biosystems 7500 User Manual

Chapter 7 Verifying the Instrument Performance
Preparing the TaqMan
®
RNase P Verification Plate
90 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Preparing the TaqMan
®
RNase P Verification Plate
IMPORTANT! You must perform an ROI calibration
(see page 35), a background calibration (see page 49)
and a pure dye calibration (see page 67) before
running an RNase P plate.
IMPORTANT! Wear powder-free
gloves when you handle the
RNase P
Verification plate .
1. Retrieve the TaqMan RNase P Verification Plate
Kit from the freezer, remove the RNase P plate,
then allow the plate to warm to room temperature
(approximately 5 minutes).
2. Remove the RNase P plate from its packaging.
Note: If the RNase P plate contains a
compression pad, remove it from the plate.
Applied Biosystems does not recommend the use
of compression pads with the 7300/7500/7500
Fast system.
3. Standard plates only: Vortex the plate for
5 seconds. Do not vortex Fast plates.
(Remaining steps apply to both standard and Fast
plates.)
4. Centrifuge the plate for 2 minutes in a centrifuge
with a plate adapter (<1500xg).
IMPORTANT! The plate must be well mixed and
centrifuged.
>00:05:00Ambient
RNase P
Verification Plate
00:00:05<1500× g

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)