Applied Biosystems 7500 User Manual

Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 49
Notes
Chapter 5
5
Performing the
Background Calibration and Optical
Calibration
Performin
g
the
Back
g
round
C
alibration
a
n
d
Op
tical
C
alibratio
n
Powerin
g
Off the
7300
/
7500
/
7500 Fast S
y
ste
m
Performing the
Pure D
y
e
C
alibratio
n
Verif
y
in
g
the
In
s
tr
u
m
e
n
t
P
e
rf
o
rm
a
n
ce
Before Startin
g
th
e
In
s
t
a
ll
a
ti
on
Performin
g
the
R
O
I
C
alibratio
n
Unpackin
g
and
Settin
g
Up the
In
s
tr
u
m
e
n
t
Installin
g
the
S
oftware and
Powerin
g
On the
In
s
tr
u
m
e
n
t
Seepage59
Seepage52
Seepage54
Performing the Optical
Calibration for the
7500/7500 Fast System
Preparing the Background
Calibration Plate
Creating a Plate Document
for the Background
Calibration
Seepage56
Analyzing the Background
Calibration Data
Seepage55
Performing the Background
Calibration
Seepage64
Troubleshooting

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)