Applied Biosystems 7500 - Performing the Pure Dye Calibration; Preparing for the Calibration; Creating a Plate Document for the Pure Dye Calibration; Preparing Plates and Performing the Pure Dye Calibration

Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 67
Notes
Chapter 6
6
Performing the Pure Dye Calibration
Performin
g
the
Pure Dye
Calibration
Powerin
g
Off the
7300
/
7500
/
7500 Fast S
y
ste
m
Verif
y
in
g
the
In
s
tr
u
m
e
n
t
P
e
rf
o
rm
a
n
ce
Before Startin
g
th
e
In
s
t
a
ll
a
ti
on
Performin
g
the
Back
g
round
C
alibration
a
n
d
O
p
tical Calibratio
n
Performing the
R
O
I
C
alibratio
n
Unpackin
g
and
Settin
g
Up the
In
s
tr
u
m
e
n
t
Installin
g
the
S
oftware and
Powerin
g
On the
In
s
tr
u
m
e
n
t
Seepage77
Seepage71
Seepage73
Seepage72
Analyzing the Pure Dye
Calibration Data
Preparing for the
Calibration
Preparing Plates and
Performing the Pure Dye
Calibration
Creating a Plate Document
for the Pure Dye Calibration
Seepage80
Evaluating Pure Dye
Calibration Spectra