Applied Biosystems 7500 User Manual

Chapter 7 Verifying the Instrument Performance
Performing the Verification Run
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 93
Notes
7
Performing the Verification Run
1. Load the plate in the instrument as described in
“Loading the Plate” on page 40.
Note: When closing
the instrument tray,
apply pressure to the
right side of the tray
and at an angle.
2. In the plate document:
a. Select the Instrument tab.
b. Click .
The instrument begins the run, which lasts 1.5 to
2 hours using a 7300/7500 Real-Time PCR
system or <40 minutes using a 7500 Fast
Real-Time PCR system.
Note: Before starting the run, the instrument
may pause (up to 10 minutes) to allow the heated
cover to reach the correct temperature.
3. When the run is complete, click .
Continue with “Analyzing the TaqMan
®
RNase P
Plate Data” on page 94.
Notched
corner
Well A1
12
GR2475
GR2475
7500
keyed corner
A1
A
B
C
D
E
F
G
H
123456789101112
A
1
7300/7500 System
Standard plate
7500 Fast System
Fast plate
Press
here
a
b
Click

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)