Applied Biosystems 7500 User Manual

Chapter 1 Before Starting the Installation
Planning the Installation
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 5
Notes
1
Planning the Installation
You can install, calibrate, and validate the Applied Biosystems 7300/7500/7500 Fast
Real-Time PCR System within one 8-hour work day. The installation does not require
your participation at all times. However, plan to spend most of your time working with
the instrument.
Recommended
Workflow
The steps you perform when you use this installation and maintenance guide, and the
times associated with the steps, are listed below.
Note: The Real-Time System Setup wizard performs most of the steps listed below
automatically, and requires less time for several steps. See the “Planning the Installation”
page in the Real-Time System Setup wizard for steps and times for the wizard.
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00:30:00
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00:10:00
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00:45:00
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00:15:00
Unpack and set up the instrument (Chapter 2):
a. Select additional hardware and software.
b. Uncrate and place the 7300/7500/7500 Fast system.
c. Check the shipped materials.
d. Set Up the 7300/7500/7500 Fast System.
Install and set up the software (Chapter 3):
a. Install the SDS Software for the 7300/7500/7500 Fast
System.
b. Power on and install drivers, update firmware if needed.
c. Perform the Instrument Function test.
Perform the ROI calibration (Chapter 4):
a. Prepare the ROI calibration plate.
b. Create a plate document.
c. Perform the ROI calibration.
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Set up the computer (see the Applied Biosystems Real-Time
PCR System Computer Setup Guide).
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Note: Some new instruments may require a firmware download. The download, which occurs automatically,
takes approximately 45 minutes.You must be present only at the start and end of the download.
See Note below
Time Required Task

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)