EasyManuals Logo

Applied Biosystems 7500 User Manual

Applied Biosystems 7500
174 pages
To Next Page IconTo Next Page
To Next Page IconTo Next Page
To Previous Page IconTo Previous Page
To Previous Page IconTo Previous Page
Page #83 background imageLoading...
Page #83 background image
Chapter 5 Performing the Background Calibration and Optical Calibration
Analyzing the Background Calibration Data
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 57
Notes
5
4. In the plate document, select the Results tab,
then select the Spectra tab.
5. Select all wells of the plate document.
6. Inspect the raw data for irregular spectral peaks
that exceed the following fluorescent standard
units (FSU):
If one or more wells produce raw spectra that
exceed the specified FSU, the background plate
or the sample block could contain a fluorescent
contaminant. Determine the source of the
contamination. See “Troubleshooting” on
page 64.
7. Select FileClose.
a
b
Click to select
all wells
Possible
contamination
Filter FSU
A, B, C, D (7300/7500) >72,000
E (7500) >90,000

Table of Contents

Other manuals for Applied Biosystems 7500

Questions and Answers:

Question and Answer IconNeed help?

Do you have a question about the Applied Biosystems 7500 and is the answer not in the manual?

Applied Biosystems 7500 Specifications

General IconGeneral
Detection MethodFluorescence
Sample Capacity96-well plate
Thermal CyclerYes
Temperature Uniformity±0.5°C
TypeReal-Time PCR System
Thermal Accuracy±0.25°C
Detection Channels5
Weight45 kg
Power Requirements100-240 V, 50/60 Hz
Dynamic Range9 logs
Reaction Volume Range10-100 µL
Temperature Range4°C to 100°C
Thermal Cycler Block96-well
SoftwareSDS Software
DetectionMultiplex (up to 5 colors)

Related product manuals