Applied Biosystems 7500 User Manual

Chapter 5 Performing the Background Calibration and Optical Calibration
Analyzing the Background Calibration Data
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 57
Notes
5
4. In the plate document, select the Results tab,
then select the Spectra tab.
5. Select all wells of the plate document.
6. Inspect the raw data for irregular spectral peaks
that exceed the following fluorescent standard
units (FSU):
If one or more wells produce raw spectra that
exceed the specified FSU, the background plate
or the sample block could contain a fluorescent
contaminant. Determine the source of the
contamination. See “Troubleshooting” on
page 64.
7. Select FileClose.
a
b
Click to select
all wells
Possible
contamination
Filter FSU
A, B, C, D (7300/7500) >72,000
E (7500) >90,000

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)